SufT is required for growth of Mycobacterium smegmatis under iron limiting conditions
作者: Tsaone TamuhlaLydia JoubertDanicke WillemseMonique J. Williams
作者单位: 1Stellenbosch University, Cape Town , South Africa
2Stellenbosch University, Stellenbosch , South Africa
刊名: Microbiology, 2019
来源数据库: Microbiology Society
DOI: 10.1099/mic.0.000881
关键词: Iron-sulphur clusterIronMycobacteriumBiofilm
原始语种摘要: Iron-sulphur (FeS) clusters are versatile cofactors required for a range of biological processes within cells. Due to the reactive nature of the constituent molecules, assembly and delivery of these cofactors requires a multi-protein machinery in vivo . In prokaryotes, SufT homologues are proposed to function in the maturation and transfer of FeS clusters to apo-proteins. This study used targeted gene deletion to investigate the role of SufT in the physiology of mycobacteria, using Mycobacterium smegmatis as a model organism. Deletion of the sufT gene in M. smegmatis had no impact on growth under standard culture conditions and did not significantly alter activity of the FeS cluster dependent enzymes succinate dehydrogenase (SDH) and aconitase (ACN). Furthermore, the Δ sufT mutant was no...
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  • required 必需
  • Mycobacterium -ria
  • conditions 条件式
  • growth 生长
  • proteins 蛋白质
  • biogenesis 生源说
  • mutant 突变体
  • sulphur 
  • limiting 限制处理
  • mycobacteria 分枝细菌