Establishment of infectious genotype 4 cell culture-derived hepatitis C virus
作者: Noriyuki WatanabeTakaya SuzukiTomoko DateHussein Aly HussanSu Su HmweHideki AizakiMasaya SugiyamaMasashi MizokamiWilliam Delaney IVGuofeng ChengMasamichi MuramatsuTakaji Wakita
作者单位: 1National Institute of Infectious Diseases, Tokyo , Japan
2National Center for Global Health and Medicine, Chiba , Japan
3Gilead Sciences, Foster City, CA , USA
刊名: Journal of General Virology, 2019
来源数据库: Microbiology Society
DOI: 10.1099/jgv.0.001378
关键词: HCVGenotype 4Virus culture
原始语种摘要: To establish infectious genotype 4a (GT4a) cell culture-derived hepatitis C virus (HCVcc), we constructed full-length ED43 and 12 mutants possessing single or double mutations that increase ED43 replicon replication, and performed cell culture after RNA transfection. Sequential long-term culture of full-length ED43 RNA-transfected cells showed increased viral production in two ED43 mutants named ED43 QK/SI and TR/SI among the tested clones. These ED43 mutants possessed a common mutation, R1405G, in the NS3 helicase region and another mutation, D2413G or V2414A, in the NS5a–NS5b cleavage site. Furthermore, serial reinfection of naïve Huh7.5.1 cells accelerated peak HCV production at an earlier time point after every infection. After the fourth infection, we found a common mutation, R1405G,...
全文获取路径: 英国微生物学会  (合作)
影响因子:3.127 (2012)

  • culture 
  • additional 追加的
  • virus 病毒
  • hepatitis 肝炎
  • seven 
  • derived 导生的
  • established 确定
  • transfection 转染
  • infectious 传染性的
  • length 距离