Application of Data-Independent Acquisition Approach to Study the Proteome Change from Early to Later Phases of Tomato Pathogenesis Responses
作者: Kai-Ting FanKuo-Hsin WangWei-Hung ChangJhih-Ci YangChing-Fang YehKai-Tan ChengSheng-Chi HungYet-Ran Chen
作者单位: 1Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan; (K.-T.F.); (K.-H.W.); (W.-H.C.); (C.-F.Y.); (K.-T.C.)
2Sustainable Chemical Science and Technology, Taiwan International Graduate Program, Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan;
3Sustainable Chemical Science and Technology, Taiwan International Graduate Program, Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010, Taiwan;
4Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan
刊名: International Journal of Molecular Sciences, 2019, Vol.20 (4)
来源数据库: Multidisciplinary Digital Publishing Institute
DOI: 10.3390/ijms20040863
关键词: Plant pathogenesis responsesData-independent acquisitionQuantitative proteomicsPseudomonas syringae
原始语种摘要: Plants and pathogens are entangled in a continual arms race. Plants have evolved dynamic defence and immune mechanisms to resist infection and enhance immunity for second wave attacks from the same or different types of pathogenic species. In addition to evolutionarily and physiological changes, plant-pathogen interaction is also highly dynamic at the molecular level. Recently, an emerging quantitative mass spectrometry-based proteomics approach named data-independent acquisition (DIA), has been developed for the analysis of the proteome in a high-throughput fashion. In this study, the DIA approach was applied to quantitatively trace the change in the plant proteome from the early to the later stage of pathogenesis progression. This study revealed that at the early stage of the...
全文获取路径: MDPI  (合作)
影响因子:2.464 (2012)

  • pathogenesis 发病机理
  • proteins 蛋白质
  • immunity 免疫
  • Data 数据
  • dynamic 动力学的
  • stage 
  • findings 结论
  • independent 独立的
  • triggered 触发的
  • defence 防御物