Comprehensive investigation on herbarium material was realized and taxonomical structure of the genus Aconitum in Slovakia has been ascertained on the base of phenetic analysis. Three new taxa (i.e. A. degenii subsp. degenii, A. moldavicum subsp. hosteanum, and A. moldavicum nothosubsp. confusum stat. nov.) were rediscovered for this region, and for one of them the new status was proposed. Morphological variation of A. anthora and its subdivision onto infraspecific taxa (subsp. anthora and subsp. jacquinii) are discussed. The new key for identification and maps of distribution are represented.