The results of anatomical study of submerged organs of Trapa natans (water chestnut) in budding-flowering stage are presented. The plants were collected along the shore of the Rusanivsky channel (left shore of Dnepr River, in Kiev). The light and laser scanning confocal microscopy were used. Anatomical structure of the submerged secondary roots, the submerged dissected leaves and stem had the signs that are typical for submerged organs of some hydrophytes. But, the roots are characterized by the peculiarity, they were photosynthesizing. Chloroplasts are revealed in root core cells. The middle number of chloroplasts in one parenchyma cell of submerged leaf was four times more than in one core cell of root. The fluorescence intensity of leaf chloroplasts was also more than that in root or... stem chloroplasts. The very developed aerenchyma was characterized for roots and submerged stem in comparison with the submerged dissected leaves, in which an aerenchyma was weak developed and it was presented by very small air space between the cells of inner layer in parenchyma.