Relative hypoxia and oxidative stress in spleen lymphocytes of immunized Balb/c mice as indicated by HIF-1α, HIF-2α, Nrf2 expression, and glutathione peroxidase activity
作者: Citra PraditiAni R. PrijantiSri W.A. JusmanMohamad Sadikin
作者单位: 1Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta
2Center of Hypoxia and Oxidative Stress Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Jakarta
刊名: Medical Journal of Indonesia, 2018, Vol.27 (4), pp.223-8
来源数据库: Medical Journal of Indonesia
DOI: 10.13181/mji.v27i4.2152
原始语种摘要: Background: Lymphocytes activated by immunization must increase their metabolism to meet the energy requirements for mitosis, differentiation, and protein synthesis, which may subject the cell to conditions of relative hypoxia and oxidative stress. This study was conducted to investigate the increase in the levels of transcription factors involved in both conditions. Methods: Male Balb/c mice were divided into the following four groups, each consisting of six animals: the control and three experimental groups. The experimental groups were immunized by injection of 0.2 ml of 2% sheep red blood cells (SRBC) suspended in phosphate-buffered saline (PBS). Lymphocytes were harvested from the spleens of each group at time intervals of 24-, 48-, and 72-h post-immunization. The buffy coat from...

  • hypoxia 氧过少
  • spleen 
  • immunization 免疫法
  • peroxidase 过氧物酶
  • oxidative 氧化的
  • protein 蛋白质
  • glutathione 谷胱甘肽
  • stress 应力
  • activity 活度
  • expression 表示