Article: Biology of HepatocellularCarcinoma are Restrained through PI3K/AKt Signaling Pathwaysand Targeted by Tumor Infiltrating Macrophage
作者: Gengbiao Chen Wei Lu
刊名: Cancer Cell Research, 2017, Vol.4 (13), pp.307-310
来源数据库: Guoyu exquisite culture Co., Ltd
原始语种摘要: To study the biology of hepatocellular carcinoma arerestrained through PI3K/AKt signaling pathways and targetedby tumor infiltrating macrophages.Methods: Mononuclear cellswere isolated from healthy adult peripheral blood by densitygradient centrifugation and induced with IL - 4 forselective activation of macrophagesin vitro.PI3K - siRNA andAKT – siRNA were transfected to hepatocellular carcinomacell line HepG2 respectively by lipofectermine 3000, andthen cultivate with selective activation of macrophages (M2)for 48 hour. PI3K and Akt mRNA expression level in livercancer HepG2 cells were detected with real-time fluorescentquantitative PCR. Results: After transfection, PI3K, and AktmRNA level were significantly decreased. In vitromicroenvironment of liver cancer, M2 can...
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  • hepatocellular 肝细胞性的
  • Article 物品
  • liver 
  • macrophages 巨噬细胞
  • carcinoma 癌症
  • significantly 较大地
  • through 经过
  • transfection 转染
  • peripheral 周边的
  • promote 助长