Measurement of extracellular pH, K(+), and lactate in ischemic heart.
作者: Marzouk Sayed A MBuck Richard PDunlap Larry AJohnson Timothy ACascio Wayne E
作者单位: 1Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA.
刊名: Analytical biochemistry, 2002, Vol.308 (1), pp.52-60
来源数据库: PubMed Journal
原始语种摘要: Simultaneous and continuous measurements of extracellular pH, potassium (K(+)), and lactate (L(-)) in ischemic rabbit papillary muscle are presented for the first time. Potentiometric pH and K(+) sensors and an amperometric lactate biosensor were used. These miniature electrodes were previously developed and individually tested for this purpose. The pH sensor was based on an iridium oxide layer electrodeposited on a planar platinum electrode fabricated on a flexible substrate. The potentiometric K(+) sensor was based on a polymeric membrane and valinomycin ionophore. The L(-) biosensor was based on lactate oxidase and an organic conducting salt polarized at 0.15V vs Ag/AgCl reference electrode. The utility of this novel analytical system to cardiovascular research was demonstrated by...
全文获取路径: PubMed  (合作)
影响因子:2.582 (2012)

  • extracellular 胞外的
  • lactate 乳酸盐
  • ischemic 福尔克曼氏麻痹
  • compartment 隔间
  • heart 心脏
  • potassium 
  • amperometric 测量电流的
  • myocardium 心肌
  • ischemia 局部贫血
  • ionophore 离子载体