Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.
作者: Tang KFu DKötter SCotter R JCantor C RKöster H
作者单位: 1Sequenom Inc., Boston, MA 02110, USA.
刊名: Nucleic acids research, 1995, Vol.23 (16), pp.3126-31
来源数据库: PubMed Journal
原始语种摘要: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.
全文获取路径: PubMed  (合作)
影响因子:8.278 (2012)

  • probes 探针
  • duplex 全双工
  • DNA Deoxyribonucleic Acid
  • spectrometry 光谱测定
  • assisted 辅助的
  • ionization 电离化
  • strand 海滨
  • several 各自
  • desorption 解吸
  • denaturation 变性