Tax is involved in up-regulation of HMGB1 expression levels by interaction with C/EBP.
作者: Zhang Chen-GuangWang HuiNiu Zhi-GuoZhang Jing-JingYin Ming-MeiGao Zhi-TaoHu Li-Hua
作者单位: 1Clinical Laboratory Medicine Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
刊名: Asian Pacific journal of cancer prevention : APJCP, 2013, Vol.14 (1), pp.359-65
来源数据库: PubMed Journal
原始语种摘要: The high mobility group box 1 (HMGB1) protein is a multifunctional cytokine-like molecule that plays an important role in the pathogenesis of tumors. In this study, real-time polymerase chain reactions and Western blot assays indicated that HMGB1 transcriptional activity and protein level are increased in Tax+-T cells (TaxP). To clarify the mechanisms, a series of HMGB1 deletion reporter plasmids (pHLuc1 to pHLuc6) were transfected into Tax--T cells (TaxN, Jurkat) and Tax+-T cells (TaxP). We found that promoter activity in Tax+-T cells to be higher than that in Tax--T cells, indicating a significant increase in pHLuc6. Bay11-7082 (NF-κB inhibitor) treatment did not block the enhancing effect. Chromatin immunoprecipitation assays revealed that Tax was retained on a HMGB1 promoter fragment...
全文获取路径: PubMed  (合作)
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关键词翻译
关键词翻译
  • expression 表示
  • involved 涉及
  • regulation 
  • interacting 相克的
  • promoter 催化剂
  • increased 增加
  • fragment 碎屑
  • reporter 报告生成程序
  • activity 活度
  • protein 蛋白质