Immobilization of FLAG-Tagged Recombinant Adeno-Associated Virus 2 onto Tissue Engineering Scaffolds for the Improvement of Transgene Delivery in Cell Transplants.
作者: Li HuaZhang Feng-LanShi Wen-JieBai Xue-JiaJia Shu-QinZhang Chen-GuangDing Wei
作者单位: 1Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
2National Institutes for Food and Drug Controls, Beijing, China.
3Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China; Department of Medical Genetics, Capital Medical University, Beijing, China.
4Department of Medical Genetics, Capital Medical University, Beijing, China.
5Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
6Department of Medical Genetics, Capital Medical University, Beijing, China; Beijing Institute of Brain Disorders, Beijing, China.
刊名: PloS one, 2015, Vol.10 (6), pp.e0129013
来源数据库: PubMed Journal
DOI: 10.1371/journal.pone.0129013
原始语种摘要: The technology of virus-based genetic modification in tissue engineering has provided the opportunity to produce more flexible and versatile biomaterials for transplantation. Localizing the transgene expression with increased efficiency is critical for tissue engineering as well as a challenge for virus-based gene delivery. In this study, we tagged the VP2 protein of type 2 adeno-associated virus (AAV) with a 3×FLAG plasmid at the N-terminus and packaged a FLAG-tagged recombinant AAV2 chimeric mutant. The mutant AAVs were immobilized onto the tissue engineering scaffolds with crosslinked anti-FLAG antibodies by N-succinimidyl-3-(2-pyridyldithiol) propionate (SPDP). Cultured cells were seeded to scaffolds to form 3D transplants, and then tested for viral transduction both in vitro and in...
全文获取路径: PubMed  (合作)
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影响因子:3.73 (2012)

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关键词翻译
关键词翻译
  • expression 表示
  • transgene 输异基因
  • targeted 对准目标的
  • tissue 组织
  • demonstrated 探明的储量
  • mutant 突变体
  • terminus 末端
  • tagged 示踪的
  • genetic 遗传的
  • engineering 工程