Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody.
作者: Yang ShunliYin ShuanghuiShang YoujunWang DiMa WeiminHe JijunGuo JianhongCai JianpingLiu Xiangtao
作者单位: 1State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China. yangshunli@caas.cn.
2State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China. yinshuanghui@caas.cn.
3State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China. shangyoujun@caas.cn.
4State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China. sdau_dw@163.com.
5State Key Laboratory of Veterinary Etiological B
刊名: BMC biotechnology, 2015, Vol.15 , pp.83
来源数据库: PubMed Journal
DOI: 10.1186/s12896-015-0201-5
原始语种摘要: BACKGROUND(#br)Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.(#br)RESULTS(#br)In this study, we developed an imunomagnetic bead based on carboxyl-magnetic beads (MNB) labeled with a single-domain antibody (sdAb) for capturing foot-and-mouth disease (FMD) Asia 1 virus. After magnetic separation, complexes of MNB-sdAb-virus were detected with either a sandwich ELISA or QDs-C5 probe under a fluorescence microscope, and the complexes were used as templates for extraction of total RNA for amplification of the VP1 or 3D gene fragments using RT-PCR and real-time RT-PCR. The Asia 1 VLPs were efficiently captured through IMNB with a high binding rate of 5.09 μg of antigen/μl of bead suspension. Moreover,...
全文获取路径: PubMed  (合作)
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影响因子:2.165 (2012)

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关键词翻译
关键词翻译
  • antibody 抗体
  • beads 
  • serotype 血清型
  • capture 捕获
  • carboxyl 羧基
  • virus 病毒
  • domain 领域
  • labeled 示踪的
  • demonstrated 探明的储量
  • single 单独的