miRNA-221 promotes proliferation, migration and invasion by targeting TIMP2 in renal cell carcinoma.
作者: Lu Guang-JianDong Yu-QianZhang Qun-MeiDi Wen-YuJiao Lu-YangGao Qing-ZuZhang Chen-Guang
作者单位: 1Clinical Laboratory, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
2Blood Transfusion Room, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
3Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
4Medical Inspection Institute, Xinxiang Medical University Xinxiang 453000, China.
刊名: International journal of clinical and experimental pathology, 2015, Vol.8 (5), pp.5224-9
来源数据库: PubMed Journal
关键词: Clear cell renal cell carcinomaTIMP2MiR-221
原始语种摘要: INTRODUCTION(#br)MicroRNAs (miRNAs) play important roles in tumorigenesis. In this study, we investigated the role of miR-221 in the development and progression of clear cell renal cell carcinoma (ccRCC).(#br)METHODS(#br)Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-221 in ccRCC tissues and cell lines. Then, we investigated the role of miR-221 to determine its potential roles on renal cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-221 and the results were validated in renal cancer cells.(#br)RESULTS(#br)In the present study, we found that miR-221 was significantly increased in ccRCC tissues and cell lines. Knocked-down expression of miR-221 remarkably inhibited...
全文获取路径: PubMed  (合作)
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关键词翻译
关键词翻译
  • migration 迁移
  • targeting 导向目标
  • renal 肾的
  • invasion 侵入
  • expression 表示
  • carcinoma 癌症
  • luciferase 荧光素酶
  • reporter 报告生成程序
  • remarkably 显著地
  • potential