Intra- and inter-isolate variation of ribosomal and protein-coding genes in Pleurotus: implications for molecular identification and phylogeny on fungal groups.
作者: He Xiao-LanLi QianPeng Wei-HongZhou JieCao Xue-LianWang DiHuang Zhong-QianTan WeiLi YuGan Bing-Cheng
作者单位: 1Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
2Jilin Agricultural University, Changchun, 130118, China.
3Mianyang Institute of Agricultural Sciences, Mianyang, 621023, China.
4Soil and Fertilizer Institute, Sichuan Academy of Agr
刊名: BMC microbiology, 2017, Vol.17 (1), pp.139
来源数据库: PubMed Journal
DOI: 10.1186/s12866-017-1046-y
关键词: Edible mushroomIntra-isolate polymorphismSpecific variation
原始语种摘要: BACKGROUND(#br)The internal transcribed spacer (ITS), RNA polymerase II second largest subunit (RPB2), and elongation factor 1-alpha (EF1α) are often used in fungal taxonomy and phylogenetic analysis. As we know, an ideal molecular marker used in molecular identification and phylogenetic studies is homogeneous within species, and interspecific variation exceeds intraspecific variation. However, during our process of performing ITS, RPB2, and EF1α sequencing on the Pleurotus spp., we found that intra-isolate sequence polymorphism might be present in these genes because direct sequencing of PCR products failed in some isolates. Therefore, we detected intra- and inter-isolate variation of the three genes in Pleurotus by polymerase chain reaction amplification and cloning in this...
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影响因子:3.104 (2012)

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关键词翻译
关键词翻译
  • variation 变异
  • isolate 隔离
  • coding 编码
  • fungal 真菌的
  • inter 
  • ribosomal 核蛋白体的
  • intraspecific 种内的
  • phylogenetic 门的
  • protein 蛋白质
  • molecular 分子的