|作者：||Antinozzi C, Marampon F, Sgrò P, Tombolini V, Lenzi A, Crescioli C, Di Luigi L|
1Unit of Endocrinology, Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135, Rome, Italy.
2Department of Radiotherapy, Sapienza University of Rome, Rome, Italy.
3Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
4Unit of Endocrinology, Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135, Rome, Italy. email@example.com.
|刊名：||Journal of endocrinological investigation, 2019, Vol.42 (8), pp.897-907|
|关键词：||Elocalcitol; Human skeletal muscle cells; Insulin; Metabolism; Testosterone;|
|原始语种摘要：||PURPOSE(#br)Skeletal muscle (Skm) plays a key role in regulating energetic metabolism through glucose homeostasis. Several hormones such as Testosterone (T) and Vitamin D (VD) have been shown to affect energy-dependent cell trafficking by determining Insulin (I)-like effects.(#br)AIM(#br)To elucidate possible hormone-related differences on muscular metabolic control, we analyzed and compared the effects of T and elocalcitol (elo), a VD analogue, on the activation of energy-dependent cell trafficking, metabolism-related-signal transduction pathways and transcription of gene downstream targets.(#br)METHODS(#br)Human fetal skeletal muscle cells (Hfsmc) treated with T or elo were analyzed for GLUT4 localization, phosphorylation/activation status of AKT, ERK1/2, IRS-1 signaling and c-MYC... protein expression.(#br)RESULTS(#br)T, similar to elo, induced GLUT4 protein translocation likely in lipid raft microdomains. While both T and elo induced a rapid IRS-1 phosphorylation, the following dynamic in phosphorylation/activation of AKT and ERK1/2 signaling was different. Moreover, T but not elo increased c-MYC protein expression.(#br)CONCLUSIONS(#br)All together, our evidence indicates that whether both T and elo are able to affect upstream I-like pathway, they differently determine downstream effects in I-dependent cascade, suggesting diverse physiological roles in mediating I-like response in human skeletal muscle.|