|作者：||Mu Hongtao, Chen Shaohuan, Liu Fengyin, Xiao Jianbo, Huang Hui, Zhang Yuhua, Sun Yuanming, Gao Xiangyang, Lei Hongtao, Yuan Xuewen|
1College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China.
2Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau Taipa, Macau.
3South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
4Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China.
5College of Food Science, South China Agricultural University, Guangzhou 510642, China.
6Guangdong Provincial Key Laboratory of Food Quali
|刊名：||Food chemistry, 2019, Vol.270 , pp.429-435|
|关键词：||Circular dichroism; Docking simulation; Fluorescence spectroscopy; Human serum albumin; Lactic acid isomers;|
|原始语种摘要：||Lactic acid enantiomers, normally found in fermented food, are absorbed into the blood and interact with plasma carrier protein human serum albumin (HSA). Unveiling the effect on the function and structure of HSA during chiral interaction can give a better understanding of the different distribution activities of the two enantiomers. Multi-spectroscopic methods and molecular modelling techniques are used to study the interactions between lactic acid enantiomers and HSA. Time-resolved and steady-state fluorescence spectra manifest that the fluorescence quenching mechanism is mainly static in type, due to complex formation. Binding interactions, deduced by thermodynamic calculation, agree with the docking prediction. Docking results and kinetic constants represent chiral-recognizing... discriminations consistently. The bindings of lactic acid enantiomers lead to some microenvironmental and slight conformational changes of HSA as shown by circular dichroism (CD), synchronous and three-dimensional fluorescence spectra. This investigation may yield useful information about the possible toxicity risk of lactic acid enantiomers to human health.|