Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application.
作者: Mu HongtaoChen ShaohuanLiu FengyinXiao JianboHuang HuiZhang YuhuaSun YuanmingGao XiangyangLei HongtaoYuan Xuewen
作者单位: 1College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China.
2Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau Taipa, Macau.
3South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
4Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China.
5College of Food Science, South China Agricultural University, Guangzhou 510642, China.
6Guangdong Provincial Key Laboratory of Food Quali
刊名: Food chemistry, 2019, Vol.270 , pp.429-435
来源数据库: PubMed Journal
DOI: 10.1016/j.foodchem.2018.07.135
关键词: Circular dichroismDocking simulationFluorescence spectroscopyHuman serum albuminLactic acid isomers
原始语种摘要: Lactic acid enantiomers, normally found in fermented food, are absorbed into the blood and interact with plasma carrier protein human serum albumin (HSA). Unveiling the effect on the function and structure of HSA during chiral interaction can give a better understanding of the different distribution activities of the two enantiomers. Multi-spectroscopic methods and molecular modelling techniques are used to study the interactions between lactic acid enantiomers and HSA. Time-resolved and steady-state fluorescence spectra manifest that the fluorescence quenching mechanism is mainly static in type, due to complex formation. Binding interactions, deduced by thermodynamic calculation, agree with the docking prediction. Docking results and kinetic constants represent chiral-recognizing...
全文获取路径: PubMed  (合作)
影响因子:3.334 (2012)

  • docking 靠泊
  • lactic 乳汁的
  • fluorescence 荧光
  • isomers 等比值线
  • understanding 理解
  • Fluorescence 荧光
  • chiral 手性的
  • serum 血清
  • interact 交互作用
  • synchronous 同时的