Vitamin D protects endothelial cells from irradiation-induced senescence and apoptosis by modulating MAPK/SirT1 axis.
作者: Marampon FGravina G LFestuccia CPopov V MColapietro E ASanità PMusio DDe Felice FLenzi AJannini E ADi Cesare ETombolini V
作者单位: 1Division of Radiotherapy and Radiobiology, Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
2Division of Radiotherapy and Radiobiology, Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. giovanniluca.gravina@libero.it.
3Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA, USA.
4Department of Radiotherapy, Policlinico Umberto I "Sapienza" University of Rome, Rome, Italy.
5Department of Radiotherapy, Policlinico Umberto I "Sapienza" University of R
刊名: Journal of endocrinological investigation, 2016, Vol.39 (4), pp.411-22
来源数据库: PubMed Journal
DOI: 10.1007/s40618-015-0381-9
关键词: EndotheliumOxidative stressRadiotherapySirT1Vitamin D
原始语种摘要: PURPOSE(#br)Radiotherapy toxicity is related to oxidative stress-mediated endothelial dysfunction. Here, we investigated on radioprotective properties of Vitamin D (Vit.D) on human endothelial cells (HUVEC).(#br)METHODS(#br)HUVEC, pre-treated with Vit.D, were exposed to ionizing radiation (IR): ROS production, cellular viability, apoptosis, senescence and western blot for protein detection were performed. The role of MAPKs pathway was investigated by using U0126 (10 μM) MEKs/ERKs-, SB203580 (2.5 μM) p38-inhibitor or by over/expressing MKK6 p38-upstream activator.(#br)RESULTS(#br)Vit.D reduced IR-induced ROS production protecting proliferating and quiescent HUVEC from cellular apoptosis or senescence, respectively, by regulating MAPKs pathways. In proliferating HUVEC, Vit.D prevented...
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影响因子:1.654 (2012)

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关键词翻译
关键词翻译
  • induced 感应的
  • senescence 衰老
  • apoptosis 细胞死
  • quiescent 
  • protein 蛋白质
  • blocked 分块
  • endothelial 内皮的
  • modulating 调制的
  • cells 麻风细胞
  • activation 活化