|作者：||Richmond O, Cecere T E, Erdogan E, Meng X J, Piñeyro P, Subramaniam S, Todd S M, LeRoith T|
1Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA, United States.
2Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA, United States. Electronic address: firstname.lastname@example.org.
|刊名：||Veterinary microbiology, 2015, Vol.180 (3-4), pp.223-9|
|关键词：||Co-infection; Monocyte derived dendritic cells (MoDC); Porcine circovirus associated disease (PCVAD); Porcine circovirus type 2 (PCV2); Porcine reproductive and respiratory syndrome virus (PRRSV); Programed cell death-1 (PD-1); Programed death ligand-1 (PD-L1);|
|原始语种摘要：||Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) continue to have a negative economic impact on global swine production operations. Host immune modulations that potentiate disease during PCV2 and/or PRRSV infections are important areas of ongoing research. In this study, we evaluated the expression levels of PD-L1, CD86, and IL-10 in order to phenotype dendritic cells following viral infection with PCV2b and/or PRRSV. The results showed that the inhibitory marker PD-L1 was significantly increased in monocyte derived dendritic cells (MoDC) in both singular PCV2 infection and PCV2/PRRSV co-infections. MoDC expression of stimulatory marker CD86 was significantly increased during singular PCV2 infections, while it was significantly decreased in... the treatment groups co-infected with both PCV2 and PRRSV. IL-10 production was highest among MoDCs that were co-infected with PCV2 and PRRSV. These results indicate that dendritic cells develop a regulatory phenotype following PCV2/PRRSV co-infections. We further investigated the role of the PD-L1/PD-1 axis in lymphocyte anergy, apoptosis, and the induction of regulatory T-cells in porcine mononuclear cell populations. Lymphocyte populations with normal PD-1 expression had higher percentages of anergic, apoptotic lymphocytes and CD4(+)CD25(HIGH)FoxP3(+) regulatory T-cells when compared to a PD-1 deficient lymphocyte population. These results implicate the PD-L1/PD-1 axis in negative regulation of lymphocyte responses in pigs.|