Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis.
作者: Niu TianshuiGuo LihuaLuo QixiaZhou KaiYu WeiChen YunboHuang ChenXiao Yonghong
作者单位: 1Collaborative Initiative Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, the First Affiliated Hospital, college of Medicine, Zhejiang University, Hangzhou, China.
2Hangzhou Red Cross Hospital/Zhe Jiang Chinese Medcine and Western Medcine Integrated Hospital, Hangzhou, China.
3Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
刊名: Virulence, 2020, Vol.11 (1), pp.1-13
来源数据库: PubMed Journal
DOI: 10.1080/21505594.2019.1700659
关键词: Acinetobacter baumanniiGene complementationGene knockoutVirulenceWza
原始语种摘要: To investigate the virulence of capsular polysaccharide export protein (Wza) in carbapenem-resistant Acinetobacter baumannii and its effect on capsule formation. wza gene knockout and complementation strains were constructed, and changes in bacterial virulence were observed using in vitro adhesion, antiserum complement killing, anti-oxidation experiments, and infections in Galleria mellonella and mice. The effect of wza knockout on the genes wzb and wzc and wzi were assessed by RT-PCR.We successfully constructed wza knockout and complementation strains. Compared with wild-type (WT) strains, wza knockout strains displayed lower adhesion to A549 cells ( p = 0.044), lower antiserum complement killing ability ( p = 0.001), and lower mortality of G. mellonella ( p = 0.010) and mice ( p =...
全文获取路径: PubMed  (合作)
影响因子:2.787 (2012)

  • knockout 出模器
  • virulence 病毒性
  • capsular 蒴果状的
  • polysaccharide 多糖类
  • antiserum 抗血清
  • adhesion 粘附
  • killing 杀伤
  • complement 补充
  • capsule 胶囊
  • returned 退回