|作者：||Wang Li, Liu Yuhui, Li Dan, Feng Shoujiang, Yang Jiangwei, Zhang Jingjing, Zhang Junlian, Wang Di, Gan Yantai|
1Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070, China.
2College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China.
3Longdong University, Qingyang, 745000, Gansu, China.
4Institute of Soil, Fertilizer and Water-saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China.
5College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, 730070, China.
6Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070, China. firstname.lastname@example.org.
|刊名：||BMC plant biology, 2019, Vol.19 (1), pp.357|
|关键词：||AtHKT1 gene; K+/Na+ ratio; Photosynthetic rate; Solanum tuberosum; Stomatal conductance; Transpiration rate;|
|原始语种摘要：||BACKGROUND(#br)Survival of plants in response to salinity stress is typically related to Na+ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.).(#br)RESULTS(#br)In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing Na+ content and improving K+/Na+ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an... quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol L- 1 NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss.(#br)CONCLUSIONS(#br)We conclude that the constitutive overexpression of AtHKT1 reduces Na+ accumulation in potato leaves and promotes the K+/Na+ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity.|