Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
作者: Alikhani NyoshaBerglund Anna-KarinEngmann TanjaSpånning ErikaVögtle F-NoraPavlov PavelMeisinger ChrisLanger ThomasGlaser Elzbieta
作者单位: 1Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Science, Stockholm University, SE-10691 Stockholm, Sweden.
刊名: Journal of molecular biology, 2011, Vol.410 (3), pp.400-10
来源数据库: PubMed Journal
DOI: 10.1016/j.jmb.2011.05.009
原始语种摘要: Mitochondrial presequences and other unstructured peptides are degraded inside mitochondria by presequence proteases (PrePs) identified in Arabidopsis thaliana (AtPreP), humans (hPreP), and yeast (Cym1/Mop112). The presequences of A. thaliana and human PreP are predicted to consist of 85 and 29 amino acids, respectively, whereas the Saccharomyces cerevisiae Cym1/Mop112 presequence contains only 7 residues. These differences may explain the reported targeting of homologous proteins to different mitochondrial subcompartments. Here we have investigated the targeting capacity of the PreP homologues' presequences. We have produced fusion constructs containing N-terminal portions of AtPreP(1-125), hPreP(1-69), and Cym1(1-40) coupled to green fluorescent protein (GFP) and studied their import...
全文获取路径: PubMed  (合作)
影响因子:3.905 (2012)

  • mitochondria 线粒体
  • conservation 保存
  • capacity 电容量
  • localization 定位
  • different 不相同的
  • species