Differential analysis of effect of high glucose level in the development of neuropathy in a tissue culture model of diabetes mellitus: role of hyperosmolality.
作者: Oztürk GErdoğan EOztürk MCengiz NHim A
作者单位: 1Yuzuncu Yil University, Medical School, Neuroscience Research Unit/Department of Physiology, Van, Turkey. drgurkan@yyu.edu.tr
刊名: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2008, Vol.116 (10), pp.582-91
来源数据库: PubMed Journal
DOI: 10.1055/s-2008-1065334
原始语种摘要: To analyse the contributions of metabolic toxicity of high glucose level and accompanying hyperosmolality to the development of diabetic neuropathy, mouse dorsal root ganglion (DRG) cultures were used. DRGs from postnatal day 7 mice were embedded in collagen gel and incubated in RPMI 1640 culture medium with increasing concentrations of glucose or equimolar amounts of mannitol which would create similar osmolalities. Outgrowth of axons from the peripheral nerve attached to DRG and migration of cells into the gel were quantified. The extent of cell death, apoptosis and mitosis among the migrating cells and apoptosis among DRG neurons following exposure to high glucose or mannitol were also evaluated. The growth of axons was almost equally affected by increasing concentrations of glucose or...
全文获取路径: PubMed  (合作)
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关键词翻译
关键词翻译
  • glucose 葡萄糖
  • neuropathy 神经病
  • mannitol 甘露糖醇
  • diabetes 糖尿病
  • extent 范围
  • development 开发
  • migrating 移动的
  • DRG 阻力
  • cells 麻风细胞
  • affected 受了影响的