Two novel protein chips for the detection of antibodies against porcine parvovirus.
作者: Wu YueWu XudanHou JinxiuChen XiongnanHuang XiaoboZhou Bin
作者单位: 1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
2College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
3College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
4MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. zhoubin@njau.edu.cn.
刊名: BMC veterinary research, 2020, Vol.16 (1), pp.57
来源数据库: PubMed Journal
DOI: 10.1186/s12917-020-02280-z
关键词: Antibody detectionClinical serumPorcine parvovirus (PPV)Protein chip
原始语种摘要: BACKGROUND(#br)PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complement-fixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies.(#br)RESULTS(#br)We establish the visible protein chip and the cyanine dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of...
全文获取路径: PubMed  (合作)
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来源刊物:
影响因子:1.861 (2012)

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关键词翻译
关键词翻译
  • protein 蛋白质
  • chips 碎石
  • parvovirus 微细小病毒
  • detection 探测
  • antibody 抗体
  • serum 血清
  • diluted 稀释的
  • visible 看得见的
  • neutralization 中和
  • PPV Peak to Peak Voltage