Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat.
作者: Naqvi Muhammad Ali-Ul-HusnainAimulajiang KalibixiatiMemon Muhammad AliHasan Muhammad WaqqasNaqvi Sana ZahraLakho Shakeel AhmedChu WenXu LixinSong XiaokaiLi XiangruiYan Ruofeng
作者单位: 1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, No.1, Weigang, Nanjing, Jiangsu Province, People's Republic of China, 210095.
2MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural Unive
刊名: BMC veterinary research, 2020, Vol.16 (1), pp.36
来源数据库: PubMed Journal
DOI: 10.1186/s12917-020-2261-6
关键词: Cold shock domain containing proteinEarly diagnosisHaemonchus contortusImmunoblottingIndirect-ELISA
原始语种摘要: BACKGROUND(#br)Haemonchus contortus (H. contortus) is one of the most important parasites that cause huge economic losses to small ruminant industry worldwide. Effective prognosis and treatment depend upon the early diagnosis of H. contortus infection. To date, no widely-approved methods for the identification of prepatent H. contortus infection are available to identify prepatent H. contortus infection properly. The aim of this study was to evaluate the diagnostic potential of recombinant cold shock H. contortus protein (rHc-CS) during early and late infections of H. contortus in goat.(#br)RESULTS(#br)Purified rHc-CS exhibited a clear band, with a molecular weight about 38 kDa. H. contortus eggs were not detected by fecal egg count technique from feces collected at 0 to 14 days post...
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影响因子:1.861 (2012)

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关键词翻译
关键词翻译
  • antibody 抗体
  • potential 
  • protein 蛋白质
  • antigen 抗原
  • early 
  • blocking 阻塞
  • specific 
  • domain 领域
  • immunoblotting 免疫印迹
  • dilution 稀度