|作者：||van den Born Erwin, van den Elzen Paul P M, van Kilsdonk Emma, Hoeijmakers Mathieu J H, Segers Ruud P A M|
1MSD Animal Health, P.O. Box 31, 5830, AA Boxmeer, The Netherlands. email@example.com.
2MSD Animal Health, P.O. Box 31, 5830, AA Boxmeer, The Netherlands.
|刊名：||BMC veterinary research, 2020, Vol.16 (1), pp.55|
|关键词：||Mummification; Porcine parvovirus; Pregnant gilts; Vaccination;|
|原始语种摘要：||BACKGROUND(#br)Porcilis® Ery+Parvo+Lepto is an octavalent inactivated ready-to-use vaccine that contains Erysipelothrix rhusiopathiae (Ery), porcine parvovirus (PPV), and six serogroups of Leptospira (Lepto). The efficacy of Porcilis® Ery + Parvo+Lepto against reproductive problems associated with porcine parvovirus (PPV) infection was evaluated in pregnant gilts. For this, a group of ninegilts was vaccinated twice (at 5 and 6 months old) with Porcilis® Ery + Parvo+Lepto (Group 1), while a group of eight gilts was included as unvaccinated controls (Group 2). All pigs were artificially inseminated 4 weeks after the second vaccination. They were challenged during early gestation with PPV-27a, a virulent cluster D strain, and euthanized to collect their offspring by hysterectomy around day... 90 in pregnancy. Antibody responses against PPV in gilts were measured, and the presence of PPV in progeny was also determined.(#br)RESULTS(#br)No clinical signs were observed after vaccination. After PPV challenge, all foetuses from the vaccinated gilts were alive (132/132), while in the unvaccinated group only 41% were alive (46/112), 19.6% were dead and 39.4% of the offspring (44/112) were mummified. PPV could be detected by qPCR in 14% of the progeny from vaccinated gilts at an average of 4.7 log10/ml, whereas this was significantly higher in the control group, where 90% of the progeny were PPV positive, with titres of 9.8 log10/ml on average.(#br)CONCLUSIONS(#br)The present study demonstrates that vaccination of gilts with Porcilis® Ery + Parvo+Lepto was safe and induced an immune response sufficient to protect progeny against PPV by reducing transplacental infection.|