Targeted Mutagenesis of the Rice FW 2.2 -Like Gene Family Using the CRISPR/Cas9 System Reveals OsFWL4 as a Regulator of Tiller Number and Plant Yield in Rice.
利用CRISPR / Cas9系统对水稻FW 2.2 - like基因家族进行定点突变,发现OsFWL4是水稻分蘖数和单株产量的调控因子。
作者: Gao QingsongLi GangSun HuiXu MingWang HuanhuanJi JianhuiWang DiYuan CaiyongZhao Xiangxiang
作者单位: 1Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an 223300, China.
2Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai'an 223001, China.
刊名: International journal of molecular sciences, 2020, Vol.21 (3)
来源数据库: PubMed Journal
DOI: 10.3390/ijms21030809
关键词: CRISPR/Cas9FW2.2-like geneGenome editingGrain yieldOff-target effectRiceTiller number
原始语种摘要: The FW2.2 -like ( FWL ) genes encode cysteine-rich proteins with a placenta-specific 8 domain. They play roles in cell division and organ size control, response to rhizobium infection, and metal ion homeostasis in plants. Here, we target eight rice FWL genes using the CRISPR/Cas9 system delivered by Agrobacterium -mediated transformation. We successfully generate transgenic T0 lines for 15 of the 16 targets. The targeted mutations are detected in the T0 lines of all 15 targets and the average mutation rate is found to be 81.6%. Transfer DNA (T-DNA) truncation is a major reason for the failure of mutagenesis in T0 plants. T-DNA segregation analysis reveals that the T-DNA inserts in transgenic plants can be easily eliminated in the T1 generation....
全文获取路径: PubMed  (合作)
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影响因子:2.464 (2012)

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关键词翻译
关键词翻译
  • targeted 对准目标的
  • mutagenesis 突变形成
  • eight 
  • tiller 分蘖
  • generation 世代
  • yield 成品率
  • detected 找到
  • number 号码
  • inserts 镶嵌件
  • editing 编辑