|作者：||Zheng Zhendong, Zheng Yingjuan, Zhang Meiyan, Wang Jiejun, Yu Guanzhen, Fang Wenzheng|
1Department of Oncology, Changzheng Hospital, Shanghai, 200070, China.
2Department of Oncology, General Hospital of Shenyang Military Region, Shenyang, 110016, Jilin Province, China.
3Department of Oncology, The 371st Central Hospital, Xinxiang City, 453000, Henan Province, China.
4Department of Internal Medicine, Emergency Center, East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
5Department of Oncology, Changzheng Hospital, Shanghai, 200070, China. email@example.com.
6Department of Medical Oncology, Shanghai East Hospital, Shanghai, 200120, China. firstname.lastname@example.org.
7Department of Oncology, Changzheng Hospital, Shanghai, 200070, China. email@example.com.
8Department of Medical Oncology, Shanghai East Hospital, Shanghai, 200120, China. firstname.lastname@example.org.
9Department of Oncology, Fuzhou General Hospital, Fuzhou, 350025, Fujian Province, China. email@example.com.
|刊名：||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2016, Vol.37 (4), pp.4803-11|
|关键词：||Gastric carcinoma; Immunohistochemistry; Prognosis; P-4EBP1; P-ACC; P-AMPKa; P-S6; P-mTOR;|
|原始语种摘要：||Activation of AMP-activated protein kinase (AMPK) suppressed mammalian target of rapamycin (mTOR) pathway, resulting in impaired cancer cell proliferation. Two cohorts (50 and 1072 cases) of patients with resected gastric adenocarcinoma were enrolled in the study. Immunohistochemical staining for p-AMPKa, p-ACC, p-mTOR, p-S6, and p-4EBP1 was performed on the 50-patient cohort. Tissue microarray blocks containing samples from 1072 patients of Chinese ethnicity were used for the immunohistochemical detection of p-AMPKa and p-S6 levels. p-AMPK and p-ACC were frequently inactivated in both cohorts of gastric cancer samples, while p-mTOR, p-S6, and p-4EBP1 were frequently activated in the small cohort of gastric cancer. However, only levels of p-AMPKa and p-S6 were associated with the overall... survival of gastric cancer patients. In the larger 1072-patient cohort, downregulation of p-AMPKa and upregulation of p-S6 were associated with tumor progression and were independent predictors of survival after resection of primary gastric cancer. Therefore, reciprocal expression of p-AMPKa and p-S6 may be promising prognostic biomarkers in patients with gastric cancer.|