Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco.
作者: O'Donnelly KerryZhao GuangyuanPatel PriyaButt M SalmanMak Lok HangKretschmer SimonWoscholski RudigerBarter Laura M C
作者单位: 1Institute of Chemical Biology, Department of Chemistry, Imperial College, Flowers Building, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.
2Department of Chemistry, Imperial College, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK.
3Institute of Chemical Biology, Department of Chemistry, Imperial College, Flowers Building, South Kensington Campus, Exhibition Road, L
刊名: Plant methods, 2014, Vol.10 , pp.17
来源数据库: PubMed Journal
DOI: 10.1186/1746-4811-10-17
关键词: Brassica oleraceaHydrophobic interaction chromatographyPurificationRubiscoSpinacia oleracea
原始语种摘要: BACKGROUND(#br)Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is a Calvin Cycle enzyme involved in CO2 assimilation. It is thought to be a major cause of photosynthetic inefficiency, suffering from both a slow catalytic rate and lack of specificity due to a competing reaction with oxygen. Revealing and understanding the engineering rules that dictate Rubisco's activity could have a significant impact on photosynthetic efficiency and crop yield.(#br)RESULTS(#br)This paper describes the purification and characterisation of a number of hydrophobically distinct populations of Rubisco from both Spinacia oleracea and Brassica oleracea extracts. The populations were obtained using a novel and rapid purification protocol that employs hydrophobic interaction chromatography (HIC) as a...
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影响因子:2.667 (2012)

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关键词翻译
关键词翻译
  • distinct 相异的
  • photosynthetic 光合的
  • carboxylase 羧化酶
  • unaccounted 未说明的
  • enrichment 富集
  • inefficiency 低效率
  • purification 提纯
  • assimilation 岩浆的同化酌
  • chromatography 色层分离法
  • extracts 摘记