|作者：||Shi Rui, Wang Pei-Yin, Li Xin-Yi, Chen Jian-Xin, Li Yan, Zhang Xin-Zhong, Zhang Chen-Guang, Jiang Tao, Li Wen-Bin, Ding Wei, Cheng Shu-Jun|
1Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
2Department of Neurosurgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University, Henan, China.
3University of South Florida, Tampa, FL, USA.
4Beijing Key Laboratory for Tumor Invasion and Metastasis, Cancer Institute of Capital Medical University, Beijing, China.
5Department of Biochemistry, Molecular Biology Capital Medical University, Beijing, China.
6Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China.
7Beijing Key Laborator
|刊名：||Oncotarget, 2015, Vol.6 (29), pp.26971-81|
|关键词：||Cancer prognosis; Cerebrospinal fluid; Extracellular vesicles; Glioma; Hsa-miR-21;|
|原始语种摘要：||Glioma is a most common type of primary brain tumors. Extracellular vesicles, in the form of exosomes, are known to mediate cell-cell communication by transporting cell-derived proteins and nucleic acids, including various microRNAs (miRNAs). Here we examined the cerebrospinal fluid (CSF) from patients with recurrent glioma for the levels of cancer-related miRNAs, and evaluated the values for prognosis by comparing the measures of CSF-, serum-, and exosome-contained miR-21 levels. Samples from seventy glioma patients following surgery were compared with those from brain trauma patients as a non-tumor control group. Exosomal miR-21 levels in the CSF of glioma patients were found significantly higher than in the controls; whereas no difference was detected in serum-derived exosomal miR-21... expression. The CSF-derived exosomal miR-21 levels correlated with tumor spinal/ventricle metastasis and the recurrence with anatomical site preference. From additional 198 glioma tissue samples, we verified that miR-21 levels associated with tumor grade of diagnosis and negatively correlated with the median values of patient overall survival time. We further used a lentiviral inhibitor to suppress miR-21 expression in U251 cells. The results showed that the levels of miR-21 target genes of PTEN, RECK and PDCD4 were up-regulated at protein levels. Therefore, we concluded that the exosomal miR-21 levels could be demonstrated as a promising indicator for glioma diagnosis and prognosis, particularly with values to predict tumor recurrence or metastasis.|