Comparative Analysis of Some Hsps Synthesis in Leaves of Transgenic Potato Plants with Gene gox at High Temperature
作者: G.B. Borovskii I.V. Lyubushkina O.A. Borovik O.I. Grabelnych D.V. Sauchyn O.Yu. Urbanovich
刊名: Журнал стресс-физиологии и биохимии, 2015, Vol.11 (4), pp.5-10
来源数据库: Journal of Stress Physiology & Biochemistry
关键词: glucose oxidasehydrogen peroxidehigh temperatureheat stress proteinspotato
原始语种摘要: Analysis of control and transgenic plants of potato, grade Skarb, transformed by vector constructions pBI-L-GOX ( LP line) и pBI-F-GOX ( F line) with native gene of glucose oxidase gox and pBI-GOX-mod ( MOD line) with modified gene gox-mod has been carried out. It has been shown that transgenic potato plants with genes gox and gox-mod did not have fenotypic differences with control plants. It has been confirmed that there was an increase of level of hydrogen peroxide produced by reaction, which catalized by glucose oxidase in leaves of transgenic plants. Using antibodies against some heat shock proteins (Hsp101, Hsp70/Hsc70, Hsp60, Hsp17.6C-1, Hsp17.6C-2) we revealed an increased content of Hsp17.6C-2 in leaves of LP line and Hsp60 in leaves of LP and F in the morning. It has been shown...
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  • glucose 葡萄糖
  • against 反对
  • confirmed 已确认的
  • control 控制
  • oxidase 氧化酶
  • potato 马铃薯
  • Analysis 分析
  • reaction 反酌
  • carried 携带
  • proteins 蛋白质