DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR PHENYTOIN SODIUM AND PHENOBARBITONE IN BULK AND PHARMACEUTICAL DOSAGE FORM
作者: Radhika ShahRagin Shah
作者单位: 1School of Pharmacy, R.K. University, Bhavnagar Highway, Kasturbadham, Rajkot-360020, Gujarat, India. radhika.shah75@gmail.com
刊名: International Journal of Pharmacy and Pharmaceutical Sciences, 2017, Vol.9 (10), pp.224-229
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijpps.2017v9i10.19917
原始语种摘要: Objective: To develop an accurate, simple, rapid, precise, and linear RP-HPLC method for the simultaneous estimation of phenytoin sodium and phenobarbitone in tablet dosage form and validated as per ICH guidelines.Methods: The method used was a reverse phase HPLC (RP-HPLC) method using Hypersil BDS C18, (250×4.6 mm, 5 μm) column, mobile phase comprising of methanol: phosphate buffer (pH 5.0) (50:50), flow rate of 1.0 ml/min and a detection wavelength of 215 nm using a UV detector.Results: The retention time for phenytoin sodium and phenobarbitone was found to be 3.97 min and 6.90 min, respectively. The linearity of developed method was achieved in the range of 10-30 μg/ml for phenytoin sodium and 3-9 μg/ml for phenobarbitone. The detection (LOD) and quantitation (LOQ) limits were 1.44 and...
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关键词翻译
关键词翻译
  • phenobarbitone 苯巴比通
  • phenytoin 苯妥英
  • sodium 
  • simultaneous 同时的
  • precise 精密的
  • estimation 估计
  • formulation 配方
  • accurate 精确的
  • tablet 药片
  • dosage 放射剂量