VALIDATION METHODS FOR THE ANALYSIS OF HYDROXYPROLINE FROM COLLAGEN UNDENATURED TYPE II COLLAGEN USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FLUORESCENCE
作者: Baitha Palanggatan MaggadaniHarmita .Milza Lubnan
作者单位: 1Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.. igakadeharmita@gmail.com
刊名: International Journal of Applied Pharmaceutics, 2018, Vol.10 (1), pp.403-406
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijap.2018.v10s1.89
原始语种摘要: Objective: This study aimed to validate an high-performance liquid chromatography method for analyzing undenatured Type II collagen preparationsusing a fluorescence detector.Methods: Based on the optimum analysis conditions, the compound was detected at an excitation wavelength of 255 nm and an emission wavelengthof 320 nm. The optimum mobile phase was determined to be acetate (pH 4.2) and acetonitrile (60:40) with a flow rate of 1.0 ml/min. Hydroxyprolineis a compound that does not have chromophore moiety; thus, it has to be derivatized first using 9-fluorenylmetoxycarbonyl-chloride.Results: The developed method was validated with linearity and an equation of y=3,249,704 x+141,945,072, with a value of r=0.9994. The detectedrange of hydroxyproline was 4–15 ppm. The limit of detection was...
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关键词翻译
关键词翻译
  • acetonitrile 乙腈
  • optimum 最适条件
  • fluorescence 荧光
  • liquid 液体
  • moiety 一部分
  • hydroxyproline 羟基脯氨酸
  • chloride 氯化物
  • performance 性能
  • detection 探测
  • chromophore 发色团