DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINING METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOTS
作者: Yahdiana HarahapPrastiwi Arum SariCatur Jatmika
作者单位: 1Bioavailability and Bioequivalence Laboratory, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.. yahdiana03@yahoo.com
刊名: International Journal of Applied Pharmaceutics, 2018, Vol.10 (1), pp.126-129
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijap.2018.v10s1.26
原始语种摘要: Objective: This study aimed to develop an analytical method for determining metformin concentrations in human dried blood spots (DBS) usingoptimal chromatographic conditions and DBS preparation methods and validate the analytical methods.Methods: Analysis metformin HCl performed using C-18 columns (250 mm × 4.6 mm) at a column temperature of 40°C with a mobile phase acetonitrilephosphate buffer (40:60% v/v) at pH 7.0 using a flow rate of 0.8 mL/min, a photodiode array detector at a wavelength of 234 nm, and atorvastatincalcium as an internal standard. Samples were prepared by protein precipitation with 60% methanol and then drying using nitrogen gas at 60°C for15 min before reconstituting in 200 μL of the mobile phase.Results: Invalidation procedures, the present methods met the...
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关键词翻译
关键词翻译
  • range 射程
  • concentration 浓度
  • nitrogen 
  • metformin 二甲双胍
  • standard 标准
  • analytical 分析的
  • conditions 条件式
  • applied 应用的
  • criteria 准则
  • present 出席的