SIMULTANEOUS QUANTIFICATION OF TAMOXIFEN AND 4-HYDROXY-N-DESMETHYLTAMOXIFEN LEVELS IN HUMAN PLASMA BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY: DEVELOPMENT AND APPLICATION IN BREAST CANCER PATIENTS
作者: Yahdiana HarahapNathania LeonyLetare Merry Chresia SilalahiRizka Andalusia
作者单位: 1Bioavailability/Bioequivalence Laboratory, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.. yahdiana03@yahoo.com
刊名: International Journal of Applied Pharmaceutics, 2018, Vol.10 (1), pp.370-375
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijap.2018.v10s1.82
原始语种摘要: Objective: This study was aimed to develop a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodfor the simultaneous quantification of tamoxifen, endoxifen, and clomiphene (internal standard) levels in human plasma.Methods: Plasma samples from 40 patients with breast cancer were prepared through liquid–liquid extraction in ethyl acetate, and chromatographicseparation was performed on an Acquity UPLC BEH C18 column (1.7 μm, 2.1 mm×100 mm). The sample was eluted within 6.50 min at a flow rate of0.200 ml/min under a gradient of 0.2% formic acid and acetonitrile. The analytes were then quantified through triple quadrupole MS with electrosprayionization in the positive ion mode and multiple reaction monitoring.Results: The method was successfully...
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关键词翻译
关键词翻译
  • tamoxifen 他莫西芬
  • TANDEM 美)天腾公司(主要生产容错型计算机
  • acetate 醋酸盐
  • breast 胸部
  • clomiphene 克罗米酚
  • liquid 液体
  • plasma 深绿玉髓
  • tandem 串联机
  • demonstrated 探明的储量
  • acetonitrile 乙腈