DEVELOPMENT AND VALIDATION OF STABILITY INDICATING CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF SACUBITRIL AND VALSARTAN IN PHARMACEUTICAL DOSAGE FORM
作者: Shweta MishraC. J. PatelM. M. Patel
作者单位: 1Department of Pharmaceutical Analysis, Gujarat Technological University, Ahmedabad, Gujarat, India. shwetamishra821@yahoo.com
刊名: International Journal of Applied Pharmaceutics, 2017, Vol.9 (5), pp.1-8
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijap.2017v9i5.19139
原始语种摘要: Objective: This study aims to develop and validate a stability indicating HPLC method for simultaneous estimation of sacubitril and valsartan in pharmaceutical dosage form.Methods: Sacubitril and valsartan separation were achieved by LC-20 AT C18 (250 mm x 4.6 mm) column and buffer (potassium phosphate, pH 3.0): methanol (50:50) as mobile phase, at a flow rate of 1 ml/min (millilitre per minute). Detection was carried out at 224 nm (nanometer). The different HPLC experimental parameters were optimized and the method was validated according to the standard guideline. Forced degradation experiments were carried out by exposing sacubitril and valsartan standard and sample for thermal, photolytic, oxidative and acid-base hydrolytic stress conditions.Results: Retention time of sacubitril and...
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关键词翻译
关键词翻译
  • degradation 减嚣夷酌
  • photolytic 光解的
  • simultaneous 同时的
  • standard 标准
  • milliliter 毫升
  • achieved 获得的
  • tablet 药片
  • dosage 放射剂量
  • proposed 建议的
  • hydrolytic 水解的