DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF BOSENTAN IN SPIKED HUMAN PLASMA
作者: BHAVYA SRI K.MOUNIKA C. H.
作者单位: 1Department of Pharmaceutical Analysis, RBVRR Women's College of Pharmacy, Hyderabad, India. bhavya.khagga@gmail.com
刊名: International Journal of Current Pharmaceutical Research, 2019, pp.108-110
来源数据库: Innovare Academics Sciences PVT.Ltd.
DOI: 10.22159/ijcpr.2019v11i4.34937
原始语种摘要: Objective: The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma. Methods: The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness. Results: The proposed method was found to be linear in the range of 6 to 18 mg/ml. The correlation coefficient (r2) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range. Conclusion: The analytical technique presented here...
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关键词翻译
关键词翻译
  • range 射程
  • biological 生物学的
  • analytical 分析的
  • robustness 坚固性
  • developed 开发
  • acetonitrile 乙腈
  • efficient 有用的
  • acceptable 可接受的
  • accuracy 准确度
  • linearity 线性