Regulation of HtrA2 on WT1 gene expression under imatinib stimulationand its effects on the cell biology of K562 cells
作者: Lixia ZhangYan LiXiaoyan LiQing ZhangShaowei QiuQi ZhangMin WangHaiyan XingQing RaoZheng TianKejing TangJianxiang WangYingchang Mi
作者单位: 1State Key Laboratory of Experimental Hematology, Institute ofHematology and Blood Diseases Hospital, Chinese Academy of Medical Sciencesand Peking Union Medical College, Tianjin 300020, P.R. China
2State Key Laboratory of Experiment
刊名: Oncology Letters, 2017, Vol.14 (3), pp.3862-3868
来源数据库: Spandidos Journal
DOI: 10.3892/ol.2017.6628
原始语种摘要: The aim of the present study was to investigate the regulation of WilmsTumor 1 (WT1) by serine protease high-temperature requirement protein A2 (HtrA2),a member of the Htr family, in K562 cells. In addition, the study aimed to observethe effect of this regulation on cell biological functions and its associatedmechanisms. Expression of WT1 and HtrA2 mRNA, and proteins following imatiniband the HtrA2 inhibitor 5‑[5‑(2‑nitrophenyl) furfuryl iodine]‑1, 3‑diphenyl‑2‑thiobarbituricacid (UCF‑101) treatment was detected with reverse transcription‑quantitativepolymerase chain reaction and western blot analysis. Subsequent to treatment withdrugs and UCF‑101, the proliferative function of K562 cells was detected usingMTT assays, and the rate of apoptosis was detected using Annexin V with...
全文获取路径: Spandidos  (合作)
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来源刊物:
影响因子:0.237 (2012)

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关键词翻译
关键词翻译
  • cells 麻风细胞
  • protein 蛋白质
  • pathway 轨道
  • transcription 地名转写
  • expression 表示
  • blotting 浸透
  • effect 效应
  • regulatory 控制的
  • signaling 发信号
  • treatment 处理