Ecological and Molecular Observations on Anopheles Species (diptera: culicidae) Breeding in Rock Pools on Inselbergs within Kaduna State, Nigeria
作者: Obi O.A.Nock I.H.Adebote D.A.Nwosu L.C.
作者单位: 11 Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
22 Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria
33 Department of Crop and Soil Science, Faculty of Agriculture, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State, Nigeria
刊名: Molecular Entomology, 2017, Vol.8
来源数据库: Sophia Publishing Group Inc
DOI: 10.5376/me.2017.08.0001
关键词: MicrohabitatsAnophelesDipperPolymerase chain reactionInselbergs
原始语种摘要: Studies were conducted between June and October, 2013 on the species composition, relative abundance and physicochemical conditions of breeding microhabitats of Anopheles mosquitoes in rock pools within Kaduna State, Nigeria. Plastic soup ladle dipper was used to obtain representative samples of mosquitoes in 269 rock pools on 21 inselbergs. Mosquito larvae were reared to adults on yeast diet, preserved dry on silica gel and identified morphologically with the use of a stereo microscope. Extracted genomic DNA of preserved mosquito specimens were subjected to polymerase chain reaction (PCR), followed by electrophoresis to discriminate sibling species. Three physical and ten chemical properties of larval microhabitats were determined in situ and in the laboratory using standard protocols. A...
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  • 蚊科 尼日利亚
  • 双翅目 尼日利亚
  • Nigeria 尼日利亚
  • culicidae 尼日利亚
  • diptera 尼日利亚
  • within 在以内
  • preserved 保藏的
  • anopheline 灭蚊剂
  • sympatry 同域性
  • representative 代理的
  • observed 观察到的
  • genomic 染色体组的