A Modified CTAB Method for Genomic DNA Extraction from Apple Fruit
作者: Jiuying YanChangqing MaChang BoXianguang FanZheng LiYazhou YangZhengyang Zhao
作者单位: 1Northwest Agriculture & Forestry University, College of Horticulture, Yangling, 712100, China
刊名: Molecular Plant Breeding, 2018, Vol.9
来源数据库: Sophia Publishing Group Inc
DOI: 10.5376/mpb.2018.09.0005
关键词: AppleDNA extractionPolysaccharidesPolyphenols
原始语种摘要: According to the high content of polysaccharides and polyphenols in apple fruits, three modified CTAB methods were used to extract genomic DNA of ‘Granny Smith’, with young leaves as control. The results showed that the method three was the best. This method integrated multiple purification steps, adding the interferent scavenger before cell lysis, increasing the concentration of Β-mercaptoethanol and PVP to inhibite the oxidation of polyphenol, and adding 3 mol/L NaAC of 1/10 volume, which effectively removed the polysaccharides, polyphenols and other secondary metabolites. The results of ultraviolet spectrophotometer and agarose gel electrophoresis showed that DNA was high purity and good integrity. The results of ISSR primers amplification showed that the bands were clear. The results...
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  • DNA Deoxyribonucleic Acid
  • genomic 染色体组的
  • polyphenols 多酚
  • extraction 提取
  • restriction 限制
  • agarose 琼脂糖
  • concentration 浓度
  • interferent 干扰物
  • adding 求和
  • three