Isolation, Culture, Identification and Differentiation of Canine Amniotic Mesenchymal Stem Cells in Vitro
作者: Yukun ZhangLiheng BaiYan LiMoning LiuGuifang CaoDafu Zhi
作者单位: 11 Animal Tissue Embryology and Developmental Biology Laboratory, College of Life Sciences, Inner Mongolia Agricultural University, Huhhot, China
22 Inner Mongolia Maternal and Child Health Hospital, Huhhot, China
刊名: Animal Molecular Breeding, 2017, Vol.7
来源数据库: Sophia Publishing Group Inc
DOI: 10.5376/amb.2017.07.0001
关键词: CanineAmnionMesenchymal stem cellsDifferentiationVeterinary cell therapy
原始语种摘要: Recent years, people are paying more and more attention to amniotic membrane tissue which has a rich resource and no ethical restrictions. Canine amniotic mesenchymal stem cells (cAMSCs) were collected by enzyme digestion method from canine amniotic membrane tissues. The cell morphology was continuously observed at different passages and cell growth curves of passage 3 and passage 9 were drawed. Stem cell related proteins and genes were identified by immunofluorescence and RT-PCR. The multiple differentiation potential of cAMSCs was identified by osteogenesis and adipogenesis. This study showed that the cAMSCs was long fusiform and could be continuously subcultured in vitro. The immunofluorescence results showed that Vimentin and SSEA-4 were positively expressed. The RT-PCR results showed...
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  • positively 断然
  • expressed 榨出的
  • amniotic 羊膜的
  • related 有关的
  • observed 观察到的
  • proteins 蛋白质
  • osteogenesis 骨生成
  • potential 
  • ethical 道德
  • adipogenesis 脂肪生成