Fast Neutron Radiation Induced Glu-B1 Deficient Lines of an Elite Bread Wheat Variety
作者: Debbie Laudencia-Chingcuanco
作者单位: 1Crop Improvement and Genetics Research Unit, USDA-ARS WRRC, 800 Buchanan Street, Albany, CA 94710, USA
刊名: Triticeae Genomics and Genetics, 2017, Vol.8
来源数据库: Sophia Publishing Group Inc
DOI: 10.5376/tgg.2017.08.0001
关键词: WheatGluteninHMW-GSGlu-B1Bread bakingMutantBx17+By18Fast-neutron
原始语种摘要: Five isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded by the B-genome were identified from a fast-neutron radiation-mutagenized population of Summit, an elite variety of bread wheat ( Triticum aestivum L.). The mutant lines differ from the wild-type progenitor by the absence of the HMW-GS Bx17 and By18 proteins. Gene specific primers designed for Bx17 and By18 genes failed to generate the diagnostic amplicons in the mutant lines. Assays using the iSelect Illumina Wheat 90K SNP arrays revealed that the induced deletions in these lines ranged from 5.58 to 127.47 cM. Isogenic wheat lines deficient in the different HMW-GS proteins will allow the determination of the contributions of these different genes to dough quality. This new germplasm will serve...
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  • wheat 小麦
  • dough 
  • lines 线型
  • mutant 突变体
  • bread 面包
  • isogenic 等基因的
  • different 不相同的
  • variety 变种
  • population 母体
  • germplasm 种质