The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model
作者: Prabodh RisalBaik Hwan ChoKarl G. SylvesterJae-Chun KimHyoung Tae KimYeon Jun Jeong
刊名: In Vitro Cellular & Developmental Biology. Animal, 2011, Vol.47 (8), pp.526-534
来源数据库: JSTOR
原始语种摘要: Hepatocytes are an important research tool used for numerous applications. However, a short life span and a limited capacity to replicate in vitro limit the usefulness of primary hepatocyte cultures. We have hypothesized that in vivo priming of hepatocyte could make them more susceptible to growth factors in the medium for continuous proliferation in vitro. Here, a novel approach used to establish hepatocyte cell lines that included hepatocyte priming in vivo prior to culture with a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet was attempted. The cell line grew in a monolayer while maintaining a granular cytoplasm and a round nucleus. Electron microscopy displayed hepatocyte-like features including mitochondria, glycogen granules, and the presence of bile canaliculi. This cell line...
全文获取路径: JSTOR  (合作)

  • hepatocyte 肝细胞
  • liver 
  • immortal 不朽的
  • establishment 确立
  • lines 线型
  • nude 无约因
  • demonstrated 探明的储量
  • mitochondria 线粒体
  • antitrypsin 抗胰蛋白酶
  • including 包括(气导)包括……在内包括…在内