Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3
作者: Junsoo KimHaemin LeeYeon Jin RohHan ul KimDonghyuk ShinSorah KimJonghyeon SonAro LeeMinseo KimJunga ParkSeong Yun HwangKyunghwan KimYong Kwon LeeHyun Suk JungKwang Yeon HwangByung Cheon Lee
作者单位: 1College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea
2Biochemistry Laboratory, Department of Biosystems and Biotechnology, Kangwon National University, 1 Kangwondaekak-gil, Chuncheon-si, Gangwon-do 24341, Republic of Korea
3Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Frankfurt am Main, Germany
4College of Life Sciences and
刊名: IUCrJ, 2020, Vol.7 (Pt 1), pp.90-99
来源数据库: International Union of Crystallography
DOI: 10.1107/S2052252519015409
关键词: MICALActin depolymerizationCalponin-homology domainF-actinMonooxygenasesStructure determinationProtein structureRefinementX-ray crystallographyEnzyme mechanismsNational Research Foundation of Korea2018R1A1A1A05079386National Research Foundation of Korea2018M3A9F3055925National Research Foundation of Korea2017R1A2B2005666
原始语种摘要: MICAL is an oxidoreductase that participates in cytoskeleton reorganization via actin disassembly in the presence of NADPH. Although three MICALs (MICAL1, MICAL2 and MICAL3) have been identified in mammals, only the structure of mouse MICAL1 has been reported. Here, the first crystal structure of human MICAL3, which contains the flavin-containing monooxygenase (FMO) and calponin-homology (CH) domains, is reported. MICAL3 has an FAD/NADP-binding Rossmann-fold domain for monooxygenase activity like MICAL1. The FMO and CH domains of both MICAL3 and MICAL1 are highly similar in structure, but superimposition of the two structures shows a different relative position of the CH domain in the asymmetric unit. Based on kinetic analyses, the catalytic efficiency of MICAL3 dramatically increased on...
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  • monooxygenase 加一氧酶
  • homology 同源
  • flavin 黄素
  • oxidoreductase 氧化还原酶
  • domain 领域
  • relative 相对的
  • National 国民牌大客车
  • available 可供应的
  • catalytic 催化的
  • determination 测定