Molecular characterization of Duck Plague virus isolated from Bangladesh
作者: Md. Mostakin Ahamed Muhammad Tofazzal Hossain Marzia Rahman K. H. M. Nazmul Hussain Nazir Mohammad Ferdousur Rahman Khan Md. Shafiullah Parvej Wahedul Karim Ansari Meher Negar Noor-A-Alahi Chiste Khaled Bin Amin Md. Liakot Hossen Sultan Ahmed M. Bahanur Rahman
刊名: Journal of Advanced Veterinary and Animal Research, 2015, Vol.Volume 2 (Issue 3)
来源数据库: Journal of Advanced Veterinary and Animal Research
DOI: 10.5455/javar.2015.b90
关键词: gC geneIsolationIdentificationPCRPHA testDNA polymerase gene
原始语种摘要: Duck plague (DP) is the most feared duck disease in the world. For isolation, identification, molecular detection and characterization of DP virus (DPV), a total of 94 samples were collected from commercial farms (n=6) and households (n=13) from Rajshahi (n=37), Netrokona (n=35) and Mymensingh (n=22) districts of Bangladesh. The samples were processed and inoculated into 11-13 days old embryonated duck eggs for virus propagation. Virus was identified using agar gel immunodiffusion test (AGIT) and passive hemagglutination (PHA) test, and was confirmed by polymerase chain reaction (PCR) targeting DNA polymerase and gC genes, followed by sequencing. Pathogenicity tests were performed using duck embryos, ducklings and ducks. Among the 94 samples, 17 isolates were confirmed as DPV by PCR...
全文获取路径: 高级兽医和动物研究杂志  (合作)
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关键词翻译
关键词翻译
  • virus 病毒
  • isolates 单离体
  • polymerase 聚合酶
  • isolated 隔离
  • plague 鼠疫
  • confirmed 已确认的
  • PCR Page Control Register
  • collected 收帐
  • sequencing 排序
  • isolation 隔离