Reverse transcription polymerase chain reaction (RT-PCR) based detection and serotyping of FMD Virus from field samples of Gazipur, Bangladesh, and adaptation of the virus in BHK-21 cell
作者: Mohammad Ashraful Alam Marzia Rahman Md. Liakot Hossen Sultan Ahmed Md. Shafiullah Parvej Mohammad Ferdousur Rahman Khan Md. Bahanur Rahman
刊名: Journal of Advanced Veterinary and Animal Research, 2015, Vol.Volume 2 (Issue 3)
来源数据库: Journal of Advanced Veterinary and Animal Research
DOI: 10.5455/javar.2015.b88
关键词: BHK-21 cellcytopathic effectsFMDVRT-PCR
原始语种摘要: The study aimed for the detection and serotyping of Foot and Mouth Disease virus (FMDV) circulating in Kapasia Upazila, Gazipur district of Bangladesh during 2013. Twelve samples comprising of tongue epithelium (n=8) and inter digital tissue (n=4) were collected from suspected cattle, and inocula were prepared. The inocula were inoculated into confluent BHK-21 cell line for virus propagation. After 3 subsequent passages; progressive cytopathic effects (CPE) specific for FMDV i.e., rounding and flattening of cells, breaking down of the intercellular bridge and finally cell death (almost 100%) were observed; these were indicative of successful virus propagation in the cells. Viral RNA was extracted, and Reverse Transcription Polymerase Chain Reaction (RT-PCR) was performed using three sets...
全文获取路径: 高级兽医和动物研究杂志 

  • virus 病毒
  • RT-PCR 反转录PCR
  • polymerase 聚合酶
  • serotype 血清型
  • inocula 培菌液
  • intercellular 细胞间的
  • detection 探测
  • reaction 反酌
  • confluent 汇合的
  • propagation 传播