|作者：||Heba ElSayed ElZorkany, Haidan M. El-Shorbagy, Khaled Yehia Farroh, Tareq Youssef, Salwa Sabet, Taher A. Salaheldin|
1Nanotechnology and Advanced Materials Central Lab, Agricultural Research Center, Egypt.
2Department of Zoology, Faculty of Science, Cairo University, Egypt.
3Department of Photochemistry Photobiology, National Institute for Laser Enhanced Science (NILES), Cairo University, Egypt.
41-Mostafa Elsayed Center for Nanotechnology Research,British University in Egypt.2-Nanotechnology and Advanced Materials Central Lab, Agricultural Research Center, Egypt.
|刊名：||Journal of Applied Pharmaceutical Science, 2018, Vol.8 (3), pp.1-8|
|来源数据库：||Open Science Publishers LLP|
|关键词：||Confocal microscopy; Quantum dots; Silica coating; HepG2 cells; In vitro toxicity; Green;|
|原始语种摘要：||In this study, we aimed to investigate the efficiency of silica coated CdSe/ZnS nanocrystals (CdSe/ZnS-SiO2 NCs) for imaging purposes. CdSe quantum dots (QDs) were synthesized by organometallic routes and were coated with ZnS shell by injecting solutions of diethylzinc (Zn (Et) 2) and hexamethyldislathiane ((TMS) 2 S) as precursors for zinc and sulfur ions respectively. Then, CdSe/ZnS QDs were rendered water soluble by overcoating with silica using tetraethyl orthosilicate (TEOS) as a silica precursor. QDs were characterized by UV-Vis absorption, emission spectroscopy TEM, XRD and DLS. The biocompatibility of silica-coated QDs (CdSe/ZnS-SiO2) was tested by evaluating mitochondrial activity of liver hepatocellular carcinoma (HepG2) cells exposed to different concentrations of... CdSe/ZnS-SiO2. CdSe/ZnS-SiO2 cytotoxicity was evaluated by investigating DNA damage using alkaline comet assay. The intracellular uptake and localization of QDs in HepG2 cells were monitored by fluorescence imaging using Confocal Laser Scanning Microscopy (CLSM) up to eight hours. Results showed that silica coating yielded final particles size around 35 nm possessing strong luminescence property. The cytotoxicity test results showed that CdSe/ZnS-SiO2 were nontoxic at low concentrations. CLSM showed that HepG2 cells depicted fast internalization of CdSe/ZnS-SiO2 into the cells with good fluorescence emission in the cytoplasmic portion.|