Molecular identification, L-lactic acid production, and antibacterial activity of Bacillus strains isolated from soils
作者: Vasana ToliengAuttaporn BooncharoenRatthanatda NuhwaNuttha ThongchulSomboon Tanasupawat
作者单位: 1Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand
2 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
3 Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand
刊名: Journal of Applied Pharmaceutical Science, 2018, Vol.8 (10), pp.98-105
来源数据库: Open Science Publishers LLP
DOI: 10.7324/JAPS.2018.81013
关键词: BacillusRep-PCRLactic acidAntibacterial activitySoilGreen
原始语种摘要: Fourteen spore-forming bacteria isolated from soils were evaluated for their taxonomic characterization, lactic acid production and antimicrobial activity. They were belonged to the genus Bacillus and were closely related to Bacilus coagulans LMG 6326T with 97.64-98.48 % similarity, based on 16S rRNA gene analyses. Repetitive genomic element-PCR (Rep-PCR) fingerprinting using the primers sets; BOX-PCR, ERIC-PCR, GTG-PCR and REP-PCR were used to differentiate among the species. Clustering of the isolates with the PCR fingerprint dendograms obtained two groups. Group 1 consists of two isolates, JC3 and JC11 (16.67%), and the rest ten isolates (83.33%) were distributed in another groups. Based on 16S rRNA sequence analysis and Rep PCR and the phenotypic characteristics, they were classified...
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关键词翻译
关键词翻译
  • lactic 乳汁的
  • antimicrobial 杀菌剂
  • isolates 单离体
  • activity 活度
  • PCR Page Control Register
  • identification 辨认
  • antibacterial 抗细菌的
  • production 生产
  • screened 筛过的
  • distributed 分布的