Identification of sperm‐specific proteins associated with high fertility of Punjab Red and RIR x Local cross roosters with SDS‐PAGE and Immunoblotting
作者: Gurjot Kaur MaviPrem P. DubeyRanjna S. Cheema
作者单位: 1Department of Animal Genetics and Breeding Guru Angad Dev Veterinary and Animal Sciences University Ludhiana India
2 Department of Veterinary Gynaecology and Obstetrics Guru Angad Dev Veterinary and Animal Sciences University Ludhiana India
刊名: Reproduction in Domestic Animals, 2020, Vol.55 (2), pp.127-136
来源数据库: Wiley Journal
DOI: 10.1111/rda.13593
关键词: ChickenFertilityImmunoblottingSDS‐PAGESemen attributes
原始语种摘要: Abstract(#br)The present study aimed at characterization of fertility‐associated proteins in PR and RIR x Local roosters and was conducted on two generations of birds. Roosters were divided into high‐ (>50%) and low‐fertility groups (<50%) based on sperm function tests and fertility rate in both the generations. Polyclonal antibodies were raised in rabbits against sperm proteins of first generation highly fertile roosters and tested for characterization of fertility‐associated sperm proteins in the second generation of same roosters. IgG fraction against proteins (anti‐SP IgG) was reacted with sperm proteins of both high and low fertile roosters of second generation on immunoblots. SDS‐PAGE of sperm extracts of PR and RIR x Local cross breeds resulted in resolution of 12 and 23 proteins...
全文获取路径: Wiley  (合作)
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关键词翻译
关键词翻译
  • sperm 精子
  • fertility 肥度
  • proteins 蛋白质
  • fertile 肥沃的
  • associated 相关的
  • PAGE 聚丙烯酰胺凝胶电泳
  • generation 世代
  • immunoblotting 免疫印迹
  • SDS Satellite Data System
  • against 反对