Localisation and interactions of the V ipp1 protein in cyanobacteria
作者: Samantha J. BryanNigel J. BurroughsDmitriy ShevelaJianfeng YuEva RupprechtLu‐Ning LiuGiulia MastroianniQuan XueIsabel Llorente‐GarciaMark C. LeakeLutz A. EichackerDirk SchneiderPeter J. NixonConrad W. Mullineaux
作者单位: 1School of Biological and Chemical Sciences Queen Mary University of London Mile End Road London E1 4NS UK
2 Mathematics Institute and Warwick Systems Biology Centre University of Warwick Coventry CV4 7AL UK
3 Department of Mathematics and Natural Science University of Stavanger 4036 Stavanger Norway
4 Department of Life Sciences Imperial College London London SW7 2AZ UK
5 Institut für Biochemie und Molekularbiologie ZBMZ Albert‐Ludwigs‐Universität Stefan‐Meier‐Strasse 17 79104 Freiburg Germany
6 Clarendon Laboratory Department of Physics University of Oxford Parks Road Oxford OX1 3PU UK
7 Department of Physics and Astronomy University College London Gower St. London WC1E 6BT UK
8 Biological Physical Sciences Institute (BPSI) Departments of Physics and Biology University of York York YO105DD UK
9 Institut für Pharmazie und Biochemie Johannes Gutenberg‐Universität Mainz 55128 Mainz Germany
10 Department of Chemistry University of Umeå S‐90187 Umeå Sweden
11 Institute of Integrative Biology University of
刊名: Molecular Microbiology, 2014, Vol.94 (5), pp.1179-1195
来源数据库: Wiley Journal
DOI: 10.1111/mmi.12826
原始语种摘要: Summary(#br)The V ipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria S ynechocystis sp. PCC 6803 and S ynechococcus sp. PCC 7942 in which V ipp1 was tagged with green fluorescent protein at the C ‐terminus and expressed from the native chromosomal locus. There was little perturbation of function. Live‐cell fluorescence imaging shows dramatic relocalisation of V ipp1 under high light. Under low light, V ipp1 is predominantly dispersed in the cytoplasm with occasional concentrations at the outer periphery of the thylakoid membranes. High light induces V ipp1 coalescence into localised puncta within minutes, with net...
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关键词翻译
关键词翻译
  • protein 蛋白质
  • cyanobacteria 蓝藻细菌
  • fluorescent 萤光的
  • assembly 组装
  • indirectly 间接地
  • cytoplasmic 细胞质的
  • thylakoid 类囊体
  • extensive 广泛的
  • terminus 末端
  • involved 涉及