Effect of physicochemical parameters on nitrile‐hydrolyzing potentials of newly isolated nitrilase of Fusarium oxysporum f. sp. lycopercisi ED‐3
作者: Manorama Bura GohainShruti TalukdarMadhumita TalukdarArchana YadavBinod Kumar GogoiTarun Chandra BoraShashi KiranArvind Gulati
作者单位: 1Biotechnology Division CSIR–North East Institute of Science and Technology Jorhat India
2 CSIR–Institute of Himalayan Bioresource Technology Palampur India
刊名: Biotechnology and Applied Biochemistry, 2015, Vol.62 (2), pp.226-236
来源数据库: Wiley Journal
DOI: 10.1002/bab.1260
关键词: nitrilasebiotransformationFusariumpH indicatorenzyme
原始语种摘要: Abstract(#br)In recent years, nitrilases from fungus have received increasing attention, and most of the studies are performed on nitrilases of bacterial origin. Frequently used methods are based on analytical methods such as high‐performance liquid chromatography, liquid chromatography–mass spectrometry, and gas chromatography; therefore, an efficient, user friendly, and rapid method has been developed to screen nitrilase enzyme based on the principle of color change of a pH indicator. Phenol red amended with the minimal medium appears light yellow at neutral pH, which changes into pink with the formation of ammonia, indicating nitrilase activity in the reaction medium. A highly potent strain ED‐3 identified as Fusarium oxysporum f. sp. lycopercisi (specific activity 17.5 µmol/Min/mg...
全文获取路径: Wiley  (合作)
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关键词翻译
关键词翻译
  • 指示剂 无环的
  • nitrilase 腈水解酶
  • sp 足太阴脾经
  • parameters 参数
  • nitrile 
  • chromatography 色层分离法
  • activity 活度
  • hydrolyzing 水解的
  • aliphatic 无环的
  • indicator 无环的
  • heterocyclic 杂环的